alexa fluor 488 conjugated anti il 4rα (R&D Systems)
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Alexa Fluor 488 Conjugated Anti Il 4rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+4r%CE%B1/Human+IL-4R+alpha+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm41376564-65-41-45
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Cell Culture:Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis Article Snippet: Proliferation was assessed by WST assay (Roche, USA, Cat. No.11644807001) ( ). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, IFN-γ, TNFα, or 4ng/ml TGF-β, for 24 hours with or without 2 hr pre-treatment with 400 ng/ml Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis. Article Snippet: Proliferation was assessed by WST assay (Roche, USA, Cat. No.11644807001) (S2 Fig). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, IFN-γ, TNFα, or 4ng/ml TGF-β, for 24 hours with or without 2 hr pre-treatment with 400 ng/ ml Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis Article Snippet: Molecular probes used were Hs0016400_m1 for COL1α1 and Hs99999902_m1 for RPLPO (Applied Biosystems, Carlsbad, CA) ( ). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, TNFα, or 4ng/ml TGF-β, for 48 hours with or without 2 hr pre-treatment with 400 ng/ml Incubation:Article Title: Interleukin-3 stabilizes CD124/IL-4α surface expression in mast cells via Tyk2 and STAT6. Article Snippet: Upon gel separation, proteins were transferred onto nitrocellulose membranes (biostep) by electroblotting. .. Membranes were blocked with dry milk and incubated with antibodies against Phospho-Tyr641-Stat6 (clone D8S9Y), Stat6 (clone D3H4), Phospho-Thr202/Tyr204-p44/42 MAPK (ERK1/2, clone E10), p44/42 MAPK (ERK1/2, clone L34F12), PhosphoTyr1054/1055-Tyk2 (cat# 9321), Tyk2 (clone E9H4T), HSP90 (cat# 4874) or β-actin (clone 8H10D10) (all from Cell Signaling) or Formalin-fixed Paraffin-Embedded:Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis. Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with Labeling:Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis. Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with Polymer:Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis. Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with |
![(A, B) Flow cytometric analysis of <t>IL-4Rα+</t> cells on splenic B220+ B cells. (C) Mice were injured using a 0.2 cm diameter punch. Wound healing and ear thickness were measured using a caliper. Ear thickness represents means ± SEM of caliper measurements. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=4. (D, E) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and <t>Il4ra</t> –/– skin. n=4. (F) Schematic of mice treated with MC903 (2 nmol per ear) or 100% ethanol for 2 weeks. (G, H) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin with treatment of MC903 or EtOH. n=5. (I) Caliper measurement of ear thickness. Points represent means ± SEM of ear fold measurements. n=4. (J) Histological staining of ear tissue cross-sections using H&E staining after MC903 or EtOH treatment. (Scale bar = 500 μm). (K) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin of WT or Il4ra –/– mice with MC903 or EtOH treatment. n=6.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3438/pmc11343438/pmc11343438__nihms-2016943-f0001.jpg)