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alexa fluor 488 conjugated anti il 4rα  (R&D Systems)


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    Structured Review

    R&D Systems alexa fluor 488 conjugated anti il 4rα
    Alexa Fluor 488 Conjugated Anti Il 4rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+4r%CE%B1/Human+IL-4R+alpha+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm41376564-65-41-45
    Average 93 stars, based on 1 article reviews
    alexa fluor 488 conjugated anti il 4rα - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Article Snippet: Proliferation was assessed by WST assay (Roche, USA, Cat. No.11644807001) ( ). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, IFN-γ, TNFα, or 4ng/ml TGF-β, for 24 hours with or without 2 hr pre-treatment with 400 ng/ml IL-4Rα (MAB230, R&D Systems) or 40 ng/ml IL-13Rα1 (AF152, R&D Systems) antibodies. ..

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis.
    Article Snippet: Proliferation was assessed by WST assay (Roche, USA, Cat. No.11644807001) (S2 Fig). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, IFN-γ, TNFα, or 4ng/ml TGF-β, for 24 hours with or without 2 hr pre-treatment with 400 ng/ ml IL-4Rα (MAB230, R&D Systems) or 40 ng/ml IL-13Rα1 (AF152, R&D Systems) antibodies. ..

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Article Snippet: Molecular probes used were Hs0016400_m1 for COL1α1 and Hs99999902_m1 for RPLPO (Applied Biosystems, Carlsbad, CA) ( ). .. Cells were cultured as described above and supplemented with vehicle or 20ng/ml IL-4, IL-13, TNFα, or 4ng/ml TGF-β, for 48 hours with or without 2 hr pre-treatment with 400 ng/ml IL-4Rα (MAB230, R&D Systems) or 40 ng/ml IL-13Rα1 (AF152, R&D Systems) antibodies, then assessed for secreted soluble collagen types I-V using Sircol assay reagents, collagen standards, and procedures as described by the manufacturer (S1000, Life Science) ( ). .. For the detection of COLA1A and aSMA proteins, cells were plated on chamber slides coated with 10 μg/ml fibronectin (Sigma-Aldrich, St. Louis, MO) in complete media washed, switched to serum-free media, and supplemented with vehicle or 20ng/ml IL-4 or IL-13, or 4ng/ml TGF-β, for 48 hours with or without 2 hr pre-treatment with 400 ng/ml IL-4Rα (MAB230, R&D Systems) or 40 ng/ml IL-13Rα1 (AF152, R&D Systems) antibodies.

    Incubation:

    Article Title: Interleukin-3 stabilizes CD124/IL-4α surface expression in mast cells via Tyk2 and STAT6.
    Article Snippet: Upon gel separation, proteins were transferred onto nitrocellulose membranes (biostep) by electroblotting. .. Membranes were blocked with dry milk and incubated with antibodies against Phospho-Tyr641-Stat6 (clone D8S9Y), Stat6 (clone D3H4), Phospho-Thr202/Tyr204-p44/42 MAPK (ERK1/2, clone E10), p44/42 MAPK (ERK1/2, clone L34F12), PhosphoTyr1054/1055-Tyk2 (cat# 9321), Tyk2 (clone E9H4T), HSP90 (cat# 4874) or β-actin (clone 8H10D10) (all from Cell Signaling) or IL-4Rα (cat# AF530-SP, R&D Systems) overnight. .. Blocked membranes were washed (in 0.1% Tween/ TBS) and incubated with a secondary anti-rabbit-Ig or antimouse-Ig (Thermo-scientific) coupled to horse-radish peroxidase.

    Formalin-fixed Paraffin-Embedded:

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis.
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, antigoat/anti-mouse, Nichirei Biosciences INC). ..

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, anti-goat/anti-mouse, Nichirei Biosciences INC). ..

    Labeling:

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis.
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, antigoat/anti-mouse, Nichirei Biosciences INC). ..

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, anti-goat/anti-mouse, Nichirei Biosciences INC). ..

    Polymer:

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis.
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, antigoat/anti-mouse, Nichirei Biosciences INC). ..

    Article Title: The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Article Snippet: .. FFPE tissues were sectioned at five-micron thickness and labeled with IL-4Rα (MAB230, R&D Systems), IL-13Rα1 (AF152, R&D Systems), or IL-13Rα2 (AF146, R&D Systems) antibodies (1:100) using AEC solution and N-Histofine (universal immune-peroxidase polymer, anti-goat/anti-mouse, Nichirei Biosciences INC). ..



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    (A, B) Flow cytometric analysis of IL-4Rα+ cells on splenic B220+ B cells. (C) Mice were injured using a 0.2 cm diameter punch. Wound healing and ear thickness were measured using a caliper. Ear thickness represents means ± SEM of caliper measurements. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=4. (D, E) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin. n=4. (F) Schematic of mice treated with MC903 (2 nmol per ear) or 100% ethanol for 2 weeks. (G, H) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin with treatment of MC903 or EtOH. n=5. (I) Caliper measurement of ear thickness. Points represent means ± SEM of ear fold measurements. n=4. (J) Histological staining of ear tissue cross-sections using H&E staining after MC903 or EtOH treatment. (Scale bar = 500 μm). (K) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin of WT or Il4ra –/– mice with MC903 or EtOH treatment. n=6.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Diminished γδ T cells during murine allergic skin inflammation is mediated by IL-4 signaling in keratinocytes

    doi: 10.4049/jimmunol.2300629

    Figure Lengend Snippet: (A, B) Flow cytometric analysis of IL-4Rα+ cells on splenic B220+ B cells. (C) Mice were injured using a 0.2 cm diameter punch. Wound healing and ear thickness were measured using a caliper. Ear thickness represents means ± SEM of caliper measurements. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=4. (D, E) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin. n=4. (F) Schematic of mice treated with MC903 (2 nmol per ear) or 100% ethanol for 2 weeks. (G, H) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin with treatment of MC903 or EtOH. n=5. (I) Caliper measurement of ear thickness. Points represent means ± SEM of ear fold measurements. n=4. (J) Histological staining of ear tissue cross-sections using H&E staining after MC903 or EtOH treatment. (Scale bar = 500 μm). (K) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin of WT or Il4ra –/– mice with MC903 or EtOH treatment. n=6.

    Article Snippet: IL-4Rα deficient mice ( Il4ra –/– ), BALB/c and C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME).

    Techniques: Quantitation Assay, Staining, Quantitative RT-PCR

    (A-C) Flow cytometric analysis of IL-4Rα+ cells on splenic B220+ B cells. n=5. (D, E) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin. n=5. (F, G) Mice were injured using a 0.2 cm diameter punch. Wound healing and ear thickness were measured using a caliper. Ear thickness represent means ± SEM of ear fold measurements. n=5. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=5. G showed ear thickness and wound closure at Day 3. (H) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin. n=5.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Diminished γδ T cells during murine allergic skin inflammation is mediated by IL-4 signaling in keratinocytes

    doi: 10.4049/jimmunol.2300629

    Figure Lengend Snippet: (A-C) Flow cytometric analysis of IL-4Rα+ cells on splenic B220+ B cells. n=5. (D, E) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in WT and Il4ra –/– skin. n=5. (F, G) Mice were injured using a 0.2 cm diameter punch. Wound healing and ear thickness were measured using a caliper. Ear thickness represent means ± SEM of ear fold measurements. n=5. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=5. G showed ear thickness and wound closure at Day 3. (H) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin. n=5.

    Article Snippet: IL-4Rα deficient mice ( Il4ra –/– ), BALB/c and C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME).

    Techniques: Quantitation Assay, Quantitative RT-PCR

    (A) Schematic of mice treated with MC903 (2 nmol per ear) or 100% ethanol for 2 weeks. (B-D) Flow cytometric analysis of IL-4Rα+ cells from splenic B220+ B cells. n=4–5. (E, F) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in Il4ra fl/fl K14cre- (Cre-) and Il4ra fl/fl cre+ (Cre+) mice skin. n=5. (G) Ear thickness was measured using a caliper. Ear thickness represent means ± SEM of ear fold measurements. n=5. (H) Mice were injured using a 0.2 cm diameter punch and wound healing was measured using a caliper. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=4–5. (I) Histological staining of ear tissue cross-sections using H&E staining after MC903 or EtOH treatment. (Scale bar = 500 μm). (J) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin. n=3–5.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Diminished γδ T cells during murine allergic skin inflammation is mediated by IL-4 signaling in keratinocytes

    doi: 10.4049/jimmunol.2300629

    Figure Lengend Snippet: (A) Schematic of mice treated with MC903 (2 nmol per ear) or 100% ethanol for 2 weeks. (B-D) Flow cytometric analysis of IL-4Rα+ cells from splenic B220+ B cells. n=4–5. (E, F) Flow cytometric analysis and quantitation of percentages and numbers of Vγ3+ T cells in Il4ra fl/fl K14cre- (Cre-) and Il4ra fl/fl cre+ (Cre+) mice skin. n=5. (G) Ear thickness was measured using a caliper. Ear thickness represent means ± SEM of ear fold measurements. n=5. (H) Mice were injured using a 0.2 cm diameter punch and wound healing was measured using a caliper. Wound healing is expressed as a percentage of wound recovery relative to original size [(wound diameter)/(0.2cm) *100]. n=4–5. (I) Histological staining of ear tissue cross-sections using H&E staining after MC903 or EtOH treatment. (Scale bar = 500 μm). (J) Quantitative RT-PCR analysis of Flg, Ivl and Fn1 in skin. n=3–5.

    Article Snippet: IL-4Rα deficient mice ( Il4ra –/– ), BALB/c and C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME).

    Techniques: Quantitation Assay, Staining, Quantitative RT-PCR